Automated enzymatic determination of plasma free fatty acids by centrifugal analysis

Automated enzymatic determination of plasma free fatty acids by centrifugal analysis

Automated colorimetric determination of free fatty acids in biologic fluids.

HE MOST FREQUENTLY USED procedure for the determination of free fatty acids (FFA) in body fluids or biologic media is that of Dole (4). This method is based on the extraction of fatty acids with organic solvents followed by titration with dilute alkali. Despite improvements in specificity (3, 5, 6), this method is subject to error resulting from dissolved atmospheric carbon dioxide. When applie...

Semi-automated method for the determination of free fatty acids in plasma.

A semi-automated method for the determination of free fatty acids in plasma based on Mosinger's manual method (Mosinger, 1965) is described and the results of a comparison with Dole's titrimetric method (Dole, 1956) are presented. There is almost perfect correlation between the two methods, and the automated technique is simple, reliable, and significantly more accurate and precise than the tit...

Quantitative Determination of Plasma Free Fatty Acids and Triglycerides by Thin-layer Chromatography.

QUANTITATIVE ANALYSIS of lipids by elution from thinlayer chromatograms (1) usually requires milligram amounts. Densitometry on thin-layer plates has been successfully used for quantitative analysis by Zollner et al. (2) for plasma cholesterol esters and by Blank et al. (3) for mixtures of mono-, diand triglycerides, neutral and phospholipids. The relation of spot area to amount of materials af...

Microtitration of free fatty acids in plasma.

I HE MICIIODETERMIXATION of fiee fatt acids (FFA) in plasma has received colisiderable attelition in recent years (1-8). Although a riumher of different procedures Ilave beemi reported, most of them consist of 2 essential steps: tire extraction of tire FFA from plasnla with orgamlic solvents, an(1 the titration of the extracted FFA in a 2-phase system with a standard aqueous base. Various extra...